Title
Chemotaxis to Atypical Chemoattractants by Soil Bacteria
Department/School
Biology
Date
2-11-2018
Document Type
Book Chapter
Keywords
Attractant, Bacteria - metabolism, Bacterial Physiological Phenomena, Bacterial Proteins - metabolism Chemoeffector, Chemoreceptor, Chemotactic Factors - metabolism, Chemotaxis, Energy taxis, Methyl-accepting chemotaxis, protein, Methyl-Accepting Chemotaxis Proteins - metabolism, Signal Transduction, Soil Microbiology
DOI
DOI: 10.1007/978-1-4939-7577-8_21
Abstract
Although the mechanism of bacterial chemotaxis has been extensively studied in enteric bacteria, the hunt for novel and atypical chemoeffectors (in enterics and distantly-related species alike) has necessitated the modification of classic chemotaxis assays to deal with recalcitrant and potentially toxic chemicals. Here, we describe detailed protocols for the quantitative and qualitative assessment of chemotaxis responses that are categorized into short-term direct population response assays and long-term metabolism-based assays that can be used to identify novel chemoeffector molecules and the specific chemoreceptors involved. We emphasize the importance of behavior-based assays to verify the biochemical and physiological relevance of newly identified chemoeffector-receptor pairs.
Published in
Methods in Molecular Biology
Citation/Other Information
Parales, R.E. and Ditty, J.L. (2018). Chemotaxis to Atypical Chemoattractants by Soil Bacteria. In: M.D. Manson (ed.) Methods in Molecular Biology vol. 1729. Humana Press, New York.