Title

Chemotaxis to Atypical Chemoattractants by Soil Bacteria

Department/School

Biology

Date

2-11-2018

Document Type

Book Chapter

Keywords

Attractant, Bacteria - metabolism, Bacterial Physiological Phenomena, Bacterial Proteins - metabolism Chemoeffector, Chemoreceptor, Chemotactic Factors - metabolism, Chemotaxis, Energy taxis, Methyl-accepting chemotaxis, protein, Methyl-Accepting Chemotaxis Proteins - metabolism, Signal Transduction, Soil Microbiology

DOI

https://doi.org/10.1007/978-1-4939-7577-8_21

Abstract

Although the mechanism of bacterial chemotaxis has been extensively studied in enteric bacteria, the hunt for novel and atypical chemoeffectors (in enterics and distantly-related species alike) has necessitated the modification of classic chemotaxis assays to deal with recalcitrant and potentially toxic chemicals. Here, we describe detailed protocols for the quantitative and qualitative assessment of chemotaxis responses that are categorized into short-term direct population response assays and long-term metabolism-based assays that can be used to identify novel chemoeffector molecules and the specific chemoreceptors involved. We emphasize the importance of behavior-based assays to verify the biochemical and physiological relevance of newly identified chemoeffector-receptor pairs.

Published in

Methods in Molecular Biology

Citation/Other Information

Parales, R.E. and Ditty, J.L. (2018). Chemotaxis to Atypical Chemoattractants by Soil Bacteria. In: M.D. Manson (ed.) Methods in Molecular Biology vol. 1729. Humana Press, New York.

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