Comment on “Photodegradation of sulfathiazole under simulated sunlight: Kinetics, photo-induced structural rearrangement, and antimicrobial activities of photoproducts”
analysis, antibiotic, antimicrobial activity, antimicrobial susceptibility, green technology, sulfadiazine, sulfathiazole, sulfonamide, sulfonamides
Recent efforts have employed antimicrobial susceptibility assays to describe the residual antimicrobial activity of antibiotics and their transformation products in a variety of environmental processes. Some authors have evaluated the results of these assays using the minimum inhibitory concentration (MIC); however, this approach has fundamental weaknesses. To highlight best practices, this comment describes the advantages of using dose-response curves to calculate the half maximal inhibitory concentration (IC50) and the potential impacts of growth media on the antimicrobial activity of sulfonamide antibiotics. •Disadvantages of MIC-based analysis of residual antimicrobial activity.•Comparison of IC50 values is best practice for antimicrobial activity measurement.•Growth media may interfere with antimicrobial activity of sulfonamides.